Tgf beta 1 protein, human, recombinant his tag, biotinylated sinobiological, 10804h08hb is produced in hek293 cells, with high purity. R human tgfbeta 1 recombinant protein, human cellexpressed. Cell therapy systems cts recombinant human tgf beta 1 tgfb1 is a bioactive protein intended for use in cell culture applications, including pre clinical. Lyophilized tgfbeta 1 although stable at room temperature for 3 weeks, should be stored desiccated below 18c. The specific activity of recombinant human tgfbeta 1 is approximately 2.
Recombinant human transforming growth factorbeta 1, rhu. The data collected includes not only links to publications in pubmed, but also provides information about sample types, species, and experimental conditions. Tgf beta 1 protein, human, recombinant his tag sinobiological. Product recombinant human tgf beta 1 animal free and. Recombinant human tgfbeta 1 human cellexpressed, cf. Recombinant protein fragment human tgf beta 1 contains aa279390 with a gsttag. Pharmacokinetics and tissue distribution of recombinant. Transforming growth factor beta1 background information. Recombinant human tgfb1 protein leu30arg278, fused to his tag at nterminus, was expressed in human 293 cells hek293. Tgf beta 3 has been detected in human, porcine, and avian sources, mainly in cells of mesenchymal origin, suggesting a different role for this protein than for tgf beta 1 or beta 2. A detailed pharmacokinetic and tissue distribution study was performed to support the clinical development of rhtgf.
Human tgf beta 1 carrierfree recombinant protein 34834882. Tgfb1 transforming growth factor beta 1 homo sapiens human. Recombinant human tgfb1 protein, histagged tgfb36h. Western blot analysis of tgf beta 1 in reduced recombinant tgf beta 1 lane 1, ladder lane 2 and nonreduced. Antitgf beta 1 antibody tested for if, ihc, wb in human. Clonal selection and growth conditions resulted in a method for production of recombinant human tgf beta 1 at 7 mgliter in conditioned cell medium. Transforming growth factor beta 1 tgfb1 is also known as. Xenofree recombinant human tgf beta 1 huxp is expressed from human 293 cells as a mature disulfidelinked homodimer with an apparent molecular mass of 25 kda. Recombinant protein fragment human tgf beta 2 contains aa303414 with a gsttag recombinant protein fragment human tgf beta 3 contains aa301412 with a gsttag. Biotinylated human tgfbeta 1 tgfb1 protein, avitag. This protein carries a polyhistidine tag at the nterminus, followed by an avi tag. Tgf beta 4 has been detected in chick embryo chondrocytes, and its distribution in other types of cells is being investigated. The recombinant mouse tgfbeta 1 protein is derived from cho. Tgf beta 1 protein, human, recombinant his tag sinobiological, 10804h08h is produced in hek293 cells, with high purity.
This products resides on a fisher scientific gsa or va contract. Transforming growth factor beta 1 tgfb1 is also known as tgf. Miyazono 2008 cold spring harbor laboratory press p. Purified recombinant human tgf beta 1 is an extremely hydrophobic protein that adheres strongly to surfaces.
Tgfb1 tgf beta 1 protein lsg30 is a recombinant human tgfb1 tgf beta 1 produced in hek 293 cells. Tgf beta 1 protein, human, recombinant sinobiological. Tgfbeta1 protein 23 products cited in the literature. Tgf beta 1, tgf beta 2 and tgf beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid. Recombinant human latent tgfbeta 1 protein 299lt005. Latent tgfbeta binding protein an overview sciencedirect. Transforming growth factor beta1, tgfbeta1, ced, dpd1, tgfb, tgfb 1, lap, tgfb1. Antitgf beta 1 antibody a00019 if validated bosterbio. Human tgf beta 1 transforming growth factor beta 1 is a member of a superfamily of homologous, disulfidelinked, homodimeric proteins that regulate the proliferation and differentiation. Recombinant antitgf beta 1 antibody epr18163 ko tested. Animalfree recombinant human tgf beta 1 is expressed from human 293 cells as a mature disulfidelinked homodimer with an apparent molecular mass of 25 kda. Tgfb1 cts recombinant human protein thermo fisher scientific.
If you are viewing this page as a nonregistered user, the prices displayed is list price. The recombinant human tgfbeta 1 human cellexpressed protein has been validated for the following applications. Pharmacokinetics and tissue distribution of recombinant human. Prodots recombinant human tgfbeta 1 protein prd24010. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. Tgfbeta1 overexpression showed opposite functions and reduced the effects of. Tgfb1 human recombinant produced in cho cells is a glycosylated homodimeric polypeptide chain. Measured by its ability to inhibit the il4dependent proliferation of ht. The tgfb1 is purified by proprietary chromatographic techniques. Human tgf beta 1 transforming growth factor beta 1 is a member of a superfamily of homologous, disulfidelinked, homodimeric proteins that regulate the proliferation and differentiation of normal and transformed cells. Knockout tested rabbit recombinant monoclonal tgf beta 1 antibody epr18163. Tgfb1 transforming growth factor beta 1 is a protein coding gene. John varga, robert lafyatis, in rheumatology sixth edition, 2015. R mouse tgfbeta 1 recombinant protein life sciences.
Tgf beta 1 human recombinant tgfb1 protein prospec. The recombinant mouse tgfbeta 1 protein has been validated for the following applications. Tgfb1 human recombinant produced in cho cells is a glycosylated homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25. The recombinant human tgfbeta 1 human cellexpressed protein is derived from hek293. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis.
Recombinant human transforming growth factor beta rhtgf. The ec50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. Tgf beta 1, tgf beta 2 and tgf beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule. Upon reconstitution tgfbeta 1 should be stored at 4c between 27 days and for future use below 18c. Following restriction enzyme processing and ligation of synthetic oligonucleotide linkers, the gene was inserted into a plasmid and transfected into chinese hamster ovary cells. Identification of a major storage site, purification, and characterization. Recombinant human tgfbeta 1 human cellexpressed, cf 7754. Recombinant human transforming growth factorbeta with hsa transforming growth factorbeta tgf. Source spodoptera frugiperda, sf 9 baculovirusderived human tgfbeta 1 protein. These three fragment recombinant proteins were made inhouse. It was originally identified for its ability to induce phenotypic transformation of fibroblasts, and recently it has been implicated in the formation of skin tumors. The ec50 is defined as the effective concentration of the growth factor at which cell proliferation inhibition is at 50% of maximum. Tests indicate that tgf beta 1 is as efficient at inducing il17 expression in th17 cells, as cytokine purified from human platelets.
Recombinant human transforming growth factorbeta 1. Recombinant human tgfbeta 1 human cellexpressed protein. Tgfb1 protein human recombinant tgfb antigen prospec. Hek293 expressed endotoxinfree animal component free. Members of this family are known to exhibit regulatory activity in immunity and proliferation pathways. Tgfbeta 1 is involved in hematopoiesis and endothelial differentiation. The main reasons were the stability of the tgfbeta. A the biological activity of human recombinant tgfbeta 3, acf was tested by its ability to inhibit proliferation of ht2 cells in the presence of human il4. Human tgf beta 1 is a 25 kda protein with each subunit containing 112 aa residues.
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